Journal: Antioxidants

Article Title: Antioxidant Activity and Cytotoxicity Evaluation of New Catechol Hydrazinyl-Thiazole Derivatives as Potential Protectors in Retinal Degenerative Processes

doi: 10.3390/antiox14060646

Figure Lengend Snippet: Cell viability of ( a ) human retinal pigment epithelial cells (ARPE-19), ( b ) dermal fibroblasts (BJ), and ( c ) lung adenocarcinoma cells (A549). Cells were exposed to different concentrations of 5a and 5b . Data (% of the control group) are expressed as mean ± SD of three independent experiments with a minimum of three technical replicates/experiment. ** p < 0.02, * p < 0.001 vs. control (for 5a ); # p < 0.001 vs. control (for 5b ).

Article Snippet: The ARPE-19 cell line was purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany) and cultured in Dulbecco’s modified Eagle’s medium/F-12 (HAM) 1:1 (Biological Industries, Beit, Israel).

Techniques: Control

Journal: Marine Drugs

Article Title: 4-(Phenylsulfanyl) Butan-2-One Attenuates the Inflammatory Response Induced by Amyloid-β Oligomers in Retinal Pigment Epithelium Cells

doi: 10.3390/md19010001

Figure Lengend Snippet: The effects of oAβ 1-42 and 4-PSB-2 on the viability of human adult retinal pigment epithelial cell line (ARPE-19 cells). ( A ) The expression of oAβ 1-42 in ARPE-19 cells: A11 (red), oAβ marker; DAPI (blue), nucleus; Bar, 100 µm. ( B ) A dose–response curve for RPE cells stimulated with 0.1 to 10 µM solutions of oAβ 1-42 for 48 h demonstrates the mild effect of 10 µM oAβ 1-42 on the viability of ARPE-19 cells ( n = 5–6/group). ( C ) The morphology of ARPE-19 cells after 0.1 to 10 µM of oAβ 1-42 treatment compared to control; Bar, 20 µm. The irregular shapes of cell bodies and nuclei of ARPE-19 cells (black arrows) and small vesicles in the cytoplasm (white arrows) were observed after oAβ 1-42 treatment. ( D ) Chemical structure of 4-PSB-2. ( E ) ARPE-19 cells were treated with 0.1% dimethyl sulfoxide (DMSO) in control, 4-PSB-2 (1, 25, 50, 100, and 200 µM) for 24 h. MTT analysis showed that 4-PSB-2 was not toxic to ARPE-19 cells, and 25 µM of 4-PSB-2 significantly increased cell viability.(* indicates p ≤ 0.001 between the groups).

Article Snippet: A human retinal pigment epithelial cell line (ARPE-19) was provided by Rong-Kung Tsai at Institute of Medical Sciences, Tzu Chi University (Taiwan), and cultured in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F12), containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO 2 and 95% air.

Techniques: Expressing, Marker, Control

Journal: Cancers

Article Title: Therapeutic Potential of Antibody-Drug Conjugate-Based Therapy in Head and Neck Cancer: A Systematic Review

doi: 10.3390/cancers13133126

Figure Lengend Snippet: Characteristics of preclinical studies.

Article Snippet: EDC22 , CD147 , Na/K-ATPase inhibitor , Non cleavable heterobifunctional linker , HNC , HNC cell lines: FaDu, OSC-19, Cal27, SCC-1 HNC xenograft models: SCC-1HNC orthotopic models: OSC-19 , Centrose, LLC , Sweeny 2013 [ ] , .

Techniques: Transfection

Journal: Cell stem cell

Article Title: Large-Scale Profiling Reveals the Influence of Genetic Variation on Gene Expression in Human Induced Pluripotent Stem Cells

doi: 10.1016/j.stem.2017.03.009

Figure Lengend Snippet: eQTL Functional Annotation Enrichments. -log10 Fisher exact enrichment p-values for 4,616 eQTL lead SNVs/indels in (A) Roadmap Epigenomics DNase hypersensitivity sites (DHSs) and (B) ENCODE DHSs. The replicate H1 hESC DHS experiments in (B) were performed in different laboratories which may account for their different levels of enrichment. (C) Fisher exact odds ratios for ENCODE H1 hESC transcription factor CHiP-seq peaks. Color indicates whether the enrichment was significant which can vary due to the number of ChIP-seq peaks for each particular mark. See also Table S3.

Article Snippet: We obtained DHSs for the H1, H9, iPS DF 6.9, and iPS DF 19.11 cell lines from Roadmap Epigenomics and merged them into one bed file.

Techniques: Functional Assay, ChIP-sequencing

Journal: Cell stem cell

Article Title: Large-Scale Profiling Reveals the Influence of Genetic Variation on Gene Expression in Human Induced Pluripotent Stem Cells

doi: 10.1016/j.stem.2017.03.009

Figure Lengend Snippet: peQTN Characteristics. (A) Number of stem cell DHSs overlapped by eQTNs for four stem cell lines from Roadmap Epigenomics (H1, H9, iPS DF 6.9, iPS DF 19.11). (B) Number of peQTNs per eGene for 1,526 eGenes with at least one peQTN. (C) Putative eQTN for MED30 that overlaps a CEBPB ChIP-seq peak and disrupts a known CEBPB motif. Scatter plot shows -log10 association p-value from EMMAX for peQTN (purple point), other significant eQTL variants (green points) and variants not associated with MED30 expression (blue points). ENCODE H1 hESC CEBPB ChIP-seq peaks (blue rectangles) and chromHMM chromatin state predictions (multi-color track) are displayed. (D) CTCF ChIP-seq peak coverage (z-score of log10 counts) from five iPSC lines for peaks containing peQTNs predicted to disrupt CTCF binding and that had evidence of CTCF allelic bias in the ChIP-seq data. Counts are stratified by the genotype of the peQTN: 0, 1, and 2 for low, intermediate, and high predicted binding of CTCF, respectively. The peak coverage is significantly associated with the peQTN genotype (r=0.087, p=0.039). (E) The P1 region with the reference allele drives expression in 96% of embryos while the alternate allele leads to a complete loss of expression. (F and G) Image showing tailbud stage Ciona embryo electroporated with (F) Snail P1 (ref. allele) > GFP or (G) Snail P1 (alt. allele) > GFP. Expression can be seen in the tail muscle for the reference allele. Images were taken at the same exposure time to allow for direct comparison. See also Figure S2 and Tables S4 and S5.

Article Snippet: We obtained DHSs for the H1, H9, iPS DF 6.9, and iPS DF 19.11 cell lines from Roadmap Epigenomics and merged them into one bed file.

Techniques: ChIP-sequencing, Expressing, Binding Assay, Comparison